Paraffin Is Used In Cell Preparation To:
Paraffin embedding is a crucial step in the preparation of tissue samples for microscopic examination. It ensures that delicate biological tissues are preserved, supported, and sectioned without losing their structural integrity. The aim of this protocol is to provide instructions for paraffin embedding of fixed, cell laden constructs. Embedded samples can, among other applications, be stained for immunofluorescence and immunohistology analysis. Follow fixation protocol before starting this protocol.
Preparation of paraffin sections for immunohistochemistry. Transfer the sections onto a superfrost plus slide. Allow the slides to dry overnight and store slides at room temperature until ready for use. Move cassettes into 70% ethanol for 24 hrs. Samples can be left in 70% for long term storage, if required. Paraffin embedding involves infiltrating tissue samples with melted paraffin wax to provide a stable medium for sectioning. The process preserves tissue morphology and cellular structure, making it easier to cut thin sections for microscopic examination. The end result is a cell culture preparation that mimics the histologic processing of tissue samples, allowing the cell culture to be used as controls for tissue immunohistochemistry and in situ hybridization.
